9/26/2023 0 Comments Wen jing liAntibodies of anti‐GFP, anti‐γ‐TIP (tonoplast marker), and anti‐H +‐ATPase (plasma membrane marker) were used (e). (e–g) Microsome from whole leaf of expressed TaNRT2.5‐3B::GFP coupled with TaNAR2.1‐6B::RFP or TaNRT2.5‐3B::GFP alone were extracted and fractionated by sucrose‐density gradient centrifugation, then the different fractions were used for Western blot. (b) Left, TaNRT2.5‐3B::GFP signal middle, TaNAR2.1‐6B::RFP signal right, overlap of these two signals. (b–d) Fluorescence of green fluorescent protein (GFP) in tobacco leaf epidermal cells expressing TaNRT2.5‐3B::GFP coupled with TaNAR2.1‐6B::RFP (b), TaNRT2.5‐3B::GFP alone (c) or TaNAR2.1‐6B::RFP (d) (Bars, 20 μm). Different letters above the columns indicate statistically significant differences at the P < 0.05 level according to one‐way ANOVA. The delta‐ 15N values are shown as the mean ± SE for four oocytes. Uptake from 500 µM of 15N labelled nitrate in 6 h was used for Xenopus oocytes which were injected with water or different cRNA combinations. (a) The nitrate transport activity of different combinations of TaNRT2.5s and TaNAR2s in pH 7.5 and 5.5 relative to water‐injected controls. The nitrate transport activity and subcellular localization of TaNRT2.5. © 2019 The Authors New Phytologist © 2019 New Phytologist Trust. NAC transcriptional factor NRT2 NUE seed vigour wheat. Both genes can potentially be used to improve grain yield and NUE in wheat. The TaNAC2-NRT2.5 module has a key role in regulating grain nitrate accumulation and seed vigour. Overexpression of TaNRT2.5-3B increases seed vigour, grain nitrate concentration and yield, whereas RNA interference of TaNRT2.5 has the opposite effects. Furthermore, in the root TaNRT2.5 and TaNRT2.1 function in post-anthesis acquisition of soil nitrate. In Xenopus oocyte assays TaNRT2.5 requires a partner protein TaNAR2.1 to give nitrate transport activity, and the transporter locates to the tonoplast in a tobacco leaf transient expression system. TaNRT2.5 is expressed in developing grain, particularly the embryo and husk. Overexpressing TaNAC2-5A increases grain nitrate concentration and seed vigour by directly binding to the promoter of TaNRT2.5-3B and positively regulating its expression. A TaNAC2 regulated gene was identified that is a NRT2-type nitrate transporter TaNRT2.5 with a key role in seed vigour. A wheat nitrate-inducible NAC transcription factor, TaNAC2, plays a critical role in promoting crop growth and nitrogen use efficiency (NUE), and now its role in seed vigour is revealed. Published by Wolters Kluwer Health, Inc.Seed vigour and early establishment are important factors determining the yield of crops. The addition of puerarin to the present treatment protocol can be considered for the management of metabolic disorders and hyperandrogenism in PCOS patients.Ĭopyright © 2021 the Author(s). Furthermore, obese patients with PCOS had significantly lower systolic blood pressure, total cholesterol, and testosterone blood levels, when compared with before treatment. Significantly improved blood levels of sex hormone binding globulin (SHBG) and superoxide dismutase (SOD) were observed in patients who received the additional treatment of puerarin, regardless of their lean or obese status, while these were not observed in patients who did not receive puerarin. The baseline characteristics were analyzed using one-factor analysis of variance (ANOVA), and post-hoc was performed using the least significance difference (LSD)-t test. The normality of distribution of the data was tested using the Kolmogorov-Smirnov method. Then, the metabolic and antioxidant biomarkers were measured. Venous blood was drawn before and after treatment. In addition to the standard modality, patients in group 1 and group 3 also orally received 150 mg/d of puerarin tablets for 3 months. All patients received standard treatment (Diane-35, 1 tablet/d, orally, plus metformin, 1.5 g/d, orally). Obese patients were further randomly assigned to the obese treatment group (group 1, n = 15) and obese control group (group 1, n = 15). Women with premature ovarian failure (POF) were assigned to the obese group (body mass index ≥24 kg/m2 and waist hip ratio >0.85) or non-obese group (group 3, n = 21). This study aims to assess the therapeutic effects of a well-known component (puerarin) obtained from a Chinese herb root in patients with polycystic ovary syndrome (PCOS).
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